Intravital and postmortem laboratory diagnostics of hydrophobia clinical cases

Rabies excitant is a neurophilic virus (Rabiesvirus), regarding to Mononegavirales order, Rhabdoviridae family, Lyssavirus genus. The situation with rabies morbidity in Russia characterized as extremely unpleasant. Saliva, tear liquid, patient’s cornea prints were used the work well as, after their death, various segments of brain and salivary glands used. Additionally, antibody concentration blood serum humans passing antirabies therapy animal bites investigated. Research eye from patient A, suffered wolf bite, fluorescent method showed positive result 6 days before death. obtained results confirmed postmortem while investigation use spectroscopy enzyme-linked analysis method, ELISA, biosamples. Intravital research biosamples picked B, homeless dog precedence Virus 9 antigen was discovered on basis confocal spectroscopy, saliva samples - ELISA genome liquid demonstrated Nested Enzyme-Linked Analysis Reverse Transcription (Nested RT-PCR). diagnostics hydrophobia afterwards fluorescence biopsy. Hydrophobia injured going clinically well-expressed manner. intravital laboratory clinical cases Russian Federation considered. These bites. Both cases, caused by wild predator yet during life. Introduction into medical practice an important element differential diagnostics.